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In medical or biological research, the use of transgenic models is essential to study the regulation and function of individual genes as well as the pathogenesis of a wide variety of diseases. Genetic modification of cells and organisms is still one of the most important tools in deciphering the genome. In cell cultures, the function of individual genes can be determined in an in vitro context. In animal models, the function of these genes is studied in the context of the organism and environmental influences. Molecular technologies help to produce these transgenic model systems.
The TT provide a wide range of services for the production, rederivation, import and cryopreservation of genetically modified mice and rats. We specialize in the development of animal models using state-of-the-art gene editing technologies. In addition to the pure microinjection, we provide extensive support in the design of genetic modifications and the identification and selection of optimal founders.
The TT has recently started to offer a "full service" to produce new transgenic lines in collaboration with Dr. Breiderhoff. Mr. Breiderhoff provides his vast experience in the planning and design of the genetic modification and assists in the efficient identification of the founders.
- Pronuclear injection for the production of transgenic mice (CripR Cas, TALEN`s, lentiviral particles, etc.),
- ES-cell-based methods for the production of transgenic mice including cell culture,
- Rederivation of mouse lines via embryo transfer,
- Embryo production e.g. for speed congenics and rapid delivery of large numbers of animals via in vitro fertilization (IVF),
- Archiving and rederivation via cryopreserved embryos and sperm,
- Establishment of new cell lines (ES cells, fibroblasts).
- Laser assisted injection microscopes,
- Biological safety cabinets for embryo transfer under SPF conditions,
- Biological safety cabinets and incubators for cell culture,
- Fluorescence microscope for imaging of labeled cells and embryos.
Viral Core Facility
The Viral Core Facility offers the processes and services required for the manufacture of viral particles for use in gene transfer targeting various tissues. These particles make it easier for researchers to study the function and properties of specific proteins as, following their introduction into specific cells by viral vectors, they can be overexpressed or downregulated.
- Advice on suitable gene transfer systems
- Production of user-specific gene transfer vectors (plasmids)
- Production of viral particles for applications at different scales, both in situ and in vivo (adenoviruses, adeno-associated viruses, retroviruses, lentiviruses, Semliki Forest viruses, and Rabies viruses)
- Quantification of viral particles (titration in cell culture, qPCR of the viral genome)
- Viral particle efficiency testing (qPCR on transcript expression, protein expression analysis using Western blot technique)
- S2 and S1 cell culture laboratories
- Cell culture microscope (Olympus)
- Cell culture incubators
- Roche LightCycler 480
- Fusion FX Chemiluminescence Detection System