The goal of this project is the development of a software module (DLL) for the quantitative comparison of dose-effect curves that were experimentally identified in different cell systems in the EU project "ACuteTox". This module is the core of an analysis software, which is being developed at the University Hospital La Fe in Valencia, Spain (Prof. Castell).
The project is composed of the following steps:
- Composition of interfaces between the analysis software and the module to be developed. Definition of the data exchange formats. Creation of a test example and checkout of the usability of the appointed interfaces.
- Development of a DLL with the following performance features:
- Import of experimental dose-effect-curves for two experiments
- Adjustment of mathematical functions (theoretical curves) to the experimental data with the same method, which is used for the "Phototox" program that was also developed by our group
- Consideration of the data variance through the pre-settable number of bootstrap replicates
- Comparison of the calculated bootstrap curves for both experiments based on the parameters PIF and MPG
- Calculation of the level of confidence (p value) for both parameters
- Generation of an output file with the calculated bootstrap curve, the parameters for curve comparison, and the corresponding confidence intervals
- Testing and improvement of the developed DLL