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DFG-Forschergruppe FOR 721/2 Molekulare Struktur und Funktion der Tight Junction |
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Teilprojekt 1 |
Prof. Dr. Michael Fromm
, Priv.-Doz.
Dr. Salah Amasheh
Institut für Klinische Physiologie,
Campus Benjamin Franklin,
Charité, Freie Universität und Humboldt-Universität, Berlin
Charakterisierung von Tight Junction-Proteinen als Barriere- oder Kanalbildner
Dieses Teilprojekt befasst sich mit der grundsätzlichen Charakterisierung von membranständigen Tight Junction-Proteinen mittels molekularbiologischer, fluoreszenzoptischer, elektronenmikroskopischer und elektrophysiologischer Techniken. Hierzu werden je nach Eignung Überexpressions- und Silencingmethoden angewandt.
Es ist geplant, die Barriere- bzw. Poreneigenschaften für Solute und Wasser einzelner Claudine sowie von humanen Tricellulin-Varianten aufzuklären und Mechanismen der molekülgrößenanbhängigen Barrierefunktion der trizellulären Tight Junction zu erforschen.
Ein Projekt befasst sich mit dem Effekt von Therapeutika chronisch entzündlicher Darmerkrankungen hinsichtlich Barrierefunktion und Signaltransduktion auf Tight Junction-Proteine renaler und intestinaler Zellen. In Kooperation mit Teilprojekt 2 werden therapeutische Effekte an Biopsiepräparaten von M. Crohn- und Colitis ulcerosa-Patienten untersucht.
An endothelialen Zellen soll der Effekt von Parametern der Durchblutung auf die Expression von Tight Junction-Proteinen untersucht und die Signaltransduktion aufgeklärt werden. Schließlich befasst sich ein Projekt mit der Untersuchung von molekularen Mechanismen von potentiellen Absorptionsenhancern, die als Modulatoren des parazellulären Weges einen temporären Moleküldurchtritt durch epitheliale und endotheliale Gewebe ermöglichen.
DFG-Mitarbeiter des Teilprojekts 1
Priv.-Doz. Dr.
Rita Rosenthal
Beteiligung am Teilprojekt 1
Dr. Susanne M. Krug
Dr. Jan F. Richter
Publikationen 2010 - 2012 (FOR 721/2)
Rachow S*, Zorn-Kruppa M* (*shared first authorship), Ohnemus U, Kirschner N, Vidal-y-Sy S, von den Driesch P, Eberle J, Mildner M, Vettorazzi E, Rosenthal R, Moll I, Brandner JM (2013) Occludin is involved in adhesion, apoptosis, differentiation and Ca2+-homeostasis of human keratinocytes: implications for tumorigenesis. PLoS One #(#): e##### (IF 4.1) [19.12.12 Accepted] TP9+TP1
Kirschner N*, Rosenthal R* (*shared first authorship), Furuse M, Moll I, Fromm M, Brandner JM (201
Krug SM, Amasheh M, Dittmann I, Christoffel I, Fromm M, Amasheh S (2013, online 12.10.2012) Sodium caprate as an enhancer of macromolecule permeation across tricellular tight junctions of intestinal cells. Biomaterials 34(1): 275-282 (IF 7.4) [PubMed] [WebPage] [PDF] TP1+TPZ
Amasheh M, Luettig J, Amasheh S, Zeitz M, Fromm M, Schulzke JD (2012) Effects of quercetin on the colonic cell culture model HT-29/B6 and rat intestine in vitro. Ann. N.Y. Acad. Sci. 1258: 100-107 (IF 3.2) [PubMed] [WebPage] [PDF] (Original paper) TP2+TP1
Barmeyer C, Erko I, Fromm A, Bojarski C, Allers C, Moos V, Zeitz M, Fromm M, Schulzke JD (2012) Ion transport and barrier function are disturbed in microscopic colitis. Ann. N.Y. Acad. Sci. 1258: 143-148 (IF 3.2) [PubMed] [WebPage] [PDF] (Original paper) TP2+TP1
Hackel D, Krug SM, Sauer RS, Mousa SA, Böcker A, Pflücke D, Wrede EJ, Kistner K, Hoffmann T, Niedermirtl B, Sommer C, Bloch L, Huber O, Blasig IE, Amasheh S, Reeh PW, Fromm M, Brack A, Rittner HL (2012) Transient opening of the perineurial barrier for analgesic drug delivery. Proc. Natl. Acad. Sci. USA 109(29): E2018-E2027 (IF 9.7) [PubMed] [WebPage] [PDF] TP8+TP1+TP3+TP5
Kirschner N, Rosenthal R, Günzel D, Moll I, Brandner JM (2012) Tight junctions and differentiation – a chicken or the egg question? Exp. Dermatol. 21: 171-175 (IF 4.2) [29.02.12 In print] TP9+TP1+TP7
Krug SM, Günzel D, Conrad MP, Rosenthal R, Fromm A, Amasheh S, Schulzke JD, Fromm M (2012) Claudin-17 forms tight junction channels with distinct anion selectivity. Cell. Mol. Life Sci. 69(16): 2765-2778 (IF 6.6) [PubMed] [WebPage] [PDF] [Supplement] TP1+TP7+TP2
Markov AG, Kruglova NM, Fomina YA, Fromm M, Amasheh S (2012) Altered expression of tight junction proteins in mammary epithelium after discontinued suckling in mice. Pflügers Arch. 463(2): 391-398 [PubMed] [HTML] [PDF] TP1
Rittner HL, Amasheh S, Moshourab R, Hackel D, Yamdeu RS, Fromm M, Stein C, Brack A (2012) Modulation of tight junction proteins in the perineurium to facilitate peripheral opioid analgesia. Anesthesiology 116(6): 1323-1334 (IF 5.
Rosenthal R*, Günzel D* (*shared first authorship), Finger C, Krug SM, Richter JF, Schulzke JD, Fromm M, Amasheh S (2012) The effect of chitosan on transcellular and paracellular mechanisms in the intestinal epithelial barrier. Biomaterials 33(9): 2791-2800 (IF 7.9) [PubMed] [HTML] [PDF] [Supplement] TP1+TP7+TP2+TPZ
Rubelt MS, Amasheh S, Grobosch T, Stein C (2012) Liquid chromatography-tandem mass spectrometry for analysis of intestinal permeability of loperamide in physiological buffer. PLoS One 7(11): e48502 (print pages 1-8) (IF 4.1)
Schumann M, Günzel D, Buergel N, Richter JF, Troeger H, May C, Fromm A, Sorgenfrei D, Daum S, Bojarski C, Heyman M, Zeitz M, Fromm M, Schulzke JD 2012) Cell polarity-determining proteins Par-3 and PP-1 are involved in epithelial tight junction defects in celiac disease. Gut 61(2): 220-228 (IF 10.1) [PubMed] [HTML] [PDF] [Supplements] TP2+TP7+TP1
Schumann M, Kamel S, Pahlitzsch ML, Lebenheim L, May C, Krauss M, Hummel M, Daum S, Fromm M, Schulzke JD (2012) Defective tight junctions in refractory celiac disease. Ann. N.Y. Acad. Sci. 1258: 43-51 (IF 3.2) [PubMed] [WebPage] [PDF] (Original paper) TP2+TP1
Takahashi A*, Saito Y*, Kondoh M*° (*shared first authorship) (°corresponding author), Matsushita K, Krug SM, Suzuki H, Tsujino H, Li X, Aoyama H, Matsuhisa K, Uno T, Fromm M Hamakubo T, Yagi K (2012) Creation and biochemical analysis of a broad-specific claudin binder. Biomaterials 33(12): 3464-3474 (IF 7.9) [PubMed] [HTML with supplements] [PDF] TP1
Veshnyakova A, Krug SM, Mueller SL, Piontek J, Protze J, Fromm M, Krause G (2012) Determinants contributing to claudin ion channel formation. Ann. N.Y. Acad. Sci. 1257: 45-53 (IF 3.2) [PubMed] [WebPage] [PDF] (Original paper)
Reviews
Günzel D, Fromm M (2012) Claudins and other tight junction proteins. Comprehensive Physiology (former Handbook of Physiology) 2(3): 1819-1852 [PubMed: soon] [WebPage] [PDF] [Selected as featured article] TP7+TP1
Günzel D, Zakrzewski S, Schmid T, Pangalos M, Wiedenhoeft J, Blasse C, Ozboda C, Krug SM (2012) From TER to trans- and paracellular resistance: Lessons from impedance spectroscopy. Ann. N.Y. Acad. Sci. 1257: 142-151 (IF 3.2) [PubMed] [WebPage] [PDF] (Review) TP
Hackel D, Brack A, Fromm M, Rittner H (2012) Modulation of tight junction proteins in the perineurium for regional pain control. Ann. N.Y. Acad. Sci. 1257: 199-206 (IF 3.2) [PubMed] [WebPage] [PDF] (Review) TP8+TP1
Hering NA, Fromm M, Schulzke JD (2012) Determinants of colonic barrier function in inflammatory bowel disease and potential therapeutics. J. Physiol. 590(5): 1035-1044 (IF 5.1) [PubMed] [HTML] [PDF] (Review) TP2+TP1
Krug SM, Günzel D, Conrad MP, Lee IM, Amasheh S, Fromm M, Yu ASL (2012) Charge-selective claudin channels. Ann. N.Y. Acad. Sci. 1257: 20-28 (IF 3.2) [PubMed] [WebPage] [PDF] (Review) TP1+TP7
Rosenthal R, Heydt MS, Amasheh M, Stein C, Fromm M, Amasheh S (2012) Analysis of absorption enhancers in epithelial cell models. Ann. N.Y. Acad. Sci. 1258: 86-92 (IF 3.2) [PubMed] [WebPage] [PDF] (Review) TP1
Schulzke JD, Günzel D, John LJ, Fromm M (2012) Perspectives in tight junction research. Ann. N.Y. Acad. Sci. 1257: 1-19 (IF 3.2) [PubMed] [WebPage] [PDF] (Superinducing review) TP2+TP7+TP1
Editions
Fromm M, Schulzke JD, Volume Editors (2012)
Fromm M, Schulzke JD, Volume Editors (2012) Barriers and channels formed by tight junction proteins II. Ann. N.Y. Acad. Sci. vol. 1258: ###-###, 24 papers [In press]
Bücker R, Krug SM, Rosenthal R, Günzel D, Fromm A, Zeitz M, Chakraborty T, Fromm M, Epple HJ, Schulzke JD (2011) Aerolysin from Aeromonas hydrophila perturbs tight junction integrity and cell lesion repair in intestinal epithelial HT-29/B6 cells. J. Infect. Dis. 204 (8): 1283-1292 [PubMed] [HTML] [PDF] TP2+TP1+TP7+TPZ
Hering NA*, Andres S* (*shared first authorship), Fromm A, van Tol EA, Amasheh M, Mankertz J, Fromm M, Schulzke JD (2011) Transforming growth factor b, a whey protein component, strengthens the intestinal barrier by upregulating claudin-4 in HT-29/B6 cells. J. Nutr. 141(5): 783-789 [PubMed] [HTML] [PDF] TP2+TP1
Hering NA, Richter JF, Krug SM, Günzel D, Fromm A, Bohn E, Rosenthal R, Bücker R, Fromm M, Troeger H, Schulzke JD (2011) Yersinia enterocolitica induces epithelial barrier dysfunction through regional tight junction changes in colonic HT-29/B6 cell monolayers. Lab. Invest. 91: 310-324 [PubMed] [HTML] [PDF] [Supplement DOC] TP2+TP1+TP7
Markov AG, Voronkova MA, Volgin GN, Yablonsky PK, Fromm M, Amasheh S (2011) Tight junction proteins contribute to barrier properties in human pleura. Resp. Physiol. Neurobiol. 175(3): 331-335 [PubMed] [HTML] [PDF] TP1
Nielsen HL, Nielsen H, Ejlertsen T, Engberg J, Günzel D, Zeitz M, Hering N, Fromm M, Schulzke JD, Bücker R (2011) Oral and fecal Campylobacter concisus strains perturb barrier function by apoptosis induction in HT-29/B6 intestinal epithelial cells. Plos One 6(8): e23858 (print pages 1-8) [PubMed] [HTML] [PDF] TP2+TP7+TP1
Stumpff F, Georgi MI, Mundhenk L, Rabbani I, Fromm M, Martens H, Günzel D (2011) Sheep rumen and omasum primary cultures and source epithelia: Barrier function aligns with expression of tight junction proteins. J. Exp. Biol. 214: 2871-2882 [PubMed] [HTML] [PDF] [Supplement] TP7+TP1
Reviews
Amasheh S, Fromm M, Günzel D (2011) Claudins of intestine and nephron - a correlation of molecular tight junction structure and barrier function. Acta Physiol. 201(1): 133-140 [PubMed] [HTML] [PDF] (Review) TP1+TP7
John LJ, Fromm M, Schulzke JD (2011) Epithelial barriers in intestinal inflammation. Antioxid. Redox Signal. 15(5): 1255-1270 (IF 8.2) [PubMed] [HTML] [PDF for printing] [PDF with links] (Review) TP2+TP1
2010
Amasheh M, Fromm A, Krug SM, Amasheh S, Andres S, Zeitz M, Fromm M, Schulzke JD (2010) TNFa-induced and berberine-antagonized tight junction barrier impairment via tyrosine kinase, pAkt, and NFkB signaling. J. Cell Sci. 123(23): 4145-4155 [PubMed] [HTML] [PDF] [Supplements]
Becker S*, Wandel E* (*shared first authorship), Wobus M, Schneider R, Amasheh S, Sittig D, Kerner C, Naumann R, Hamann J, Aust G (2010) Overexpression of CD97 in intestinal epithelial cells of transgenic mice attenuates colitis by strengthening adherens junctions. Plos One 5(1): e8507 [PubMed] [HTML] [PDF]
Bücker R, Schumann M, Amasheh S, Schulzke JD (2010) Claudins in intestinal function and disease. Curr. Top. Membr. 65: 195-227 [Directory] [HTML] [PDF] (Review / book chapter)
Epple HJ, Allers K, Tröger H, Kühl
A, Erben U, Fromm M, Zeitz M, Loddenkemper C*, Schulzke JD*, Schneider
T* (*shared last authorship) (2010) Acute HIV infection induces mucosal
infiltration with CD4+ and CD8+ T cells, epithelial apoptosis, and a mucosal
barrier defect. Gastroenterology
139(4): 1289-1300 [PubMed]
[HTML]
[PDF]
[Supplement
PDF]
Kirschner N, Houdek P, Fromm M, Moll I, Brandner JM (2010) Tight junctions form a barrier in human epidermis. Eur. J. Cell Biol. 89(11): 839-842 (IF 3.6) [PubMed] [HTML] [PDF]
Markov AG*, Veshnyakova A* (*shared first authorship), Fromm M, Amasheh M, Amasheh S (2010) Segmental expression of claudin proteins correlates with tight junction barrier properties in rat intestine. J. Comp. Physiol. B 180(4): 591-598 [PubMed] [HTML] [PDF]
Milatz S, Krug SM, Rosenthal R, Günzel D, Müller D, Schulzke JD, Amasheh S*, Fromm M* (*shared last authorship) (2010) Claudin-3 acts as a sealing component of the tight junction for ions of either charge and uncharged solutes. Biochim. Biophys. Acta Biomembr. 1798: 2048-2057 [PubMed] [HTML] [PDF]
Rosenthal R, Milatz
S, Krug SM, Oelrich B, Schulzke JD, Amasheh S, Günzel D,
Fromm M (2010) Claudin-2, a component of the tight junction, forms a
paracellular water channel.. J. Cell Sci.
123(11): 1913-1921 [PubMed]
[HTML] [PDF]
[Supplement]
Westphal JK, Dörfel MJ, Krug SM,
Cording JD, Piontek J, Blasig IE, Tauber R, Fromm M, Huber O (2010)
Tricellulin forms homomeric and heteromeric tight junctional complexes.
Cell. Mol. Life Sci. 67(12): 2057-2068 [PubMed] [HTML]
[Supplement]
[PDF] Reviews
Günzel D, Krug SM, Rosenthal R, Fromm M (2010) Biophysical methods to study tight junction permeability. Curr. Top. Membr. 65: 39-78 [Directory] [HTML] [PDF] (Review / book chapter)
Award
Krug SM, Bojarski C, Fromm A, Schulzke JD, Fromm M (2010) The tight junction protein tricellulin in Crohn's disease and ulcerative colitis. Posterpreis der European Intestinal Transport Group (EITG), Salerno, Italy
Publikationen 2007 - 2009 (FOR 721/1)
Amasheh M, Grotjohann I, Amasheh S, Fromm A, Söderholm JD, Zeitz M, Fromm M, Schulzke JD (2009) Regulation of mucosal structure and barrier function in rat colon exposed to tumor necrosis factor alpha and interferon gamma in vitro: A novel model for studying the pathomechanisms of inflammatory bowel disease cytokines. Scand. J. Gastroent. 44: 1226-1235 [PubMed] [HTML] [PDF]
Amasheh S, Dullat S, Fromm M, Schulzke JD, Buhr HJ, Kroesen AJ (2009) Inflamed pouch mucosa possesses altered tight junctions indicating recurrence of inflammatory bowel disease. Int. J. Colorectal Dis. 24(10): 1149-1156 [PubMed] [HTML] [PDF]
Günzel D, Amasheh S, Pfaffenbach S, Richter JF, Kausalya PJ, Hunziker W, Fromm M (2009) Claudin-16 affects transcellular Cl- secretion in MDCK cells. J. Physiol. (Lond.) 587(15): 3777-3793 [PubMed] [HTML] [PDF] [Supplement]
Krug SM, Amasheh S, Richter JF, Milatz S, Günzel D, Westphal JK, Huber O, Schulzke JD, Fromm M (2009) Tricellulin forms a barrier to macromolecules in tricellular tight junctions without affecting ion permeability. Mol. Biol. Cell 20: 3713-3724 [PubMed] [HTML] [PDF] [Supplement text] [Supplement video]
Krug SM, Fromm M, Günzel D (2009) Two-path impedance spectroscopy for measuring paracellular and transcellular epithelial resistance. Biophys. J. 97(8): 2202-2211 [PubMed] [HTML] [PDF] [Supplement]
Amasheh S*, Milatz S* (*shared first authorship), Krug SM, Bergs M, Amasheh M, Schulzke JD, Fromm M (2009, Epub 08.11.2008) Na+ absorption defends from paracellular back-leakage by claudin-8 upregulation. Biochem. Biophys. Res. Comm. 378(1): 45-50 [PubMed] [HTML] [PDF]
Bücker R*, Troeger H* (*shared first authorship), Kleer J, Fromm M, Schulzke JD (2009) Arcobacter butzleri induces barrier dysfunction in intestinal epithelial cells. J. Infect. Dis. 200(5): 756-764 [PubMed] [HTML] [PDF]
Günzel D, Stuiver M, Kausalya PJ, Haisch L, Krug SM, Rosenthal R, Meij IC, Hunziker W, Fromm M, Müller D (2009) Claudin-10 exists in six alternatively spliced isoforms which exhibit distinct localization and function. J. Cell Sci. 122: 1507-1517 [PubMed] [HTML] [PDF]
Mankertz J*, Amasheh M* (*shared first authorship), Krug SM, Fromm A, Amasheh S, Hillenbrand B, Tavalali S, Fromm M, Schulzke JD (2009) TNFalpha up-regulates claudin-2 expression in epithelial HT-29/B6 cells via phosphatidylinositol-3-kinase signaling. Cell Tiss. Res. 336(1): 67-77 [PubMed] [HTML] [PDF]
Troeger H, Loddenkemper C, Schneider T, Schreier E, Epple HJ, Zeitz M, Fromm M, Schulzke JD (2009) Structural and functional changes of the duodenum in human norovirus infection. Gut 58: 1070-1077 [PubMed] [HTML] [PDF]
Yu ASL, Cheng MH, Angelow S, Günzel D, Kanzawa SA, Schneeberger EE, Fromm M, Coalson RD (2009) Molecular basis for cation selectivity in claudin-2-based paracellular pores: Identification of an electrostatic interaction site. J. Gen. Physiol. 133(1): 111-127 [PubMed] [HTML] [PDF]
Amasheh M, Schlichter S, Amasheh S, Mankertz J, Zeitz M, Fromm M, Schulzke JD (2008) Quercetin enhances epithelial barrier function and increases claudin-4 expression in Caco-2 cells. J. Nutr. 138(6): 1067-1073 [PubMed] [HTML] [PDF]
Epple HJ, Schneider T, Troeger H, Kunkel D, Allers K, Moos V, Amasheh M, Loddenkemper C, Fromm M, Zeitz M, Schulzke JD (2009, Epub 04.11.2008) Impairment of the intestinal barrier is evident in untreated but absent in suppressively treated HIV-infected patients. Gut 58: 220-227 [PubMed] [HTML] [PDF]
Kroesen AJ, Dullat S, Schulzke JD, Fromm M, Buhr HJ (2008) Permanently increased mucosal permeability in patients with backwashileitis after ileoanal pouch for ulcerative colitis. Scand. J. Gastroent. 43(6): 704-711 [PubMed] [HTML] [PDF]
Schumann M, Richter JF, Wedell I, Moos V, Zimmermann-Kordmann M, Schneider T, Daum S, Zeitz M, Fromm M, Schulzke JD (2008) Mechanisms of epithelial translocation of the a2-gliadin-33mer in celiac sprue. Gut 57(6): 747-754 [PubMed] [HTML] [PDF]
Markov AG, Veshnyakova A, Krug SM, Milatz S (2007) Expression of tight junction proteins in the epithelium of small intestine of the rat. Russ. J. Physiol. (Ross. Fiziol. Zh. Im. I. M. Sechenova) 93(9): 1043-1054 [PubMed]
Troeger H, Epple HJ, Schneider T, Wahnschaffe U, Ullrich R, Burchard GD, Jelinek T, Zeitz M, Fromm M, Schulzke JD (2007) Effect of chronic Giardia lamblia infection on epithelial transport and barrier function in human duodenum. Gut 56(3): 328-335 [PubMed] [HTML] [PDF]
Zeissig S, Bürgel N, Günzel D,
Richter JF, Mankertz J, Wahnschaffe U, Kroesen AJ, Zeitz M, Fromm M,
Schulzke JD (2007) Changes in expression and distribution of claudin-2, -5 and
-8 lead to discontinuous tight junctions and barrier dysfunction in active
Crohn's disease. Gut 56(1): 61-72 [PubMed]
[HTML] [PDF] Reviews
Will C, Fromm M, Müller D (2008) Claudin tight junction proteins: novel aspects in paracellular transport. Periton. Dialysis Int. 28(6): 577-584 [PubMed] [PDF]
Ann. N.Y. Acad. Sci. 1165 [Contents and Full Papers]
Fromm M, Schulzke JD, Volume Editors (2009) Molecular structure and function of the tight junction. Ann. N.Y. Acad. Sci. 1165: pp 1-346, 46 papers, ISBN 978-1-57331-749-8 [Contents and Full Papers] [Cover]
Amasheh M, Andres S, Amasheh S, Fromm M, Schulzke JD (2009) Barrier effects of nutritional factors. Ann. N.Y. Acad. Sci. 1165: 267-273 [PubMed] [HTML] [PDF]
Amasheh S, Milatz S, Krug SM, Markov AG, Günzel D, Amasheh M, Fromm M (2009) Tight junction proteins as channel formers and barrier builders: claudin-2, -5, and -8. Ann. N.Y. Acad. Sci. 1165: 211-219 [PubMed] [HTML] [PDF]
Fromm M, Krug SM, Zeissig S, Richter JF, Rosenthal R, Schulzke JD, Günzel D (2009) High resolution analysis of barrier function. Ann. N.Y. Acad. Sci. 1165: 74-81 [PubMed] [HTML] [PDF]
Günzel D, Haisch L, Pfaffenbach S, Krug SM, Milatz S, Amasheh S, Hunziker W, Müller D (2009) Claudin function in the thick ascending limb of Henle's loop. Ann. N.Y. Acad. Sci. 1165: 152-162 [PubMed] [HTML] [PDF]
Schulzke JD, Fromm M (2009) Tight junctions: Molecular structure meets function. Ann. N.Y. Acad. Sci. 1165: 1-6 [PubMed] [HTML] [PDF]
Schulzke JD*, Ploeger S* (*shared first authorship), Amasheh M, Fromm A, Zeissig S, Troeger H, Richter JF, Bojarski C, Schumann M, Fromm M (2009) Epithelial tight junctions in intestinal inflammation. Ann. N.Y. Acad. Sci. 1165: 294-300 [PubMed] [HTML] [PDF]
Promotion
Krug SM (2009) Tricellulin und seine Funktion in der trizellulären Tight Junction von Epithelzellen. Biochemie, Freie Universität Berlin. Dr. rer. nat., summa cum laude
Vorarbeiten
Amasheh S, Meiri N, Gitter AH, Schöneberg T, Mankertz J, Schulzke JD, Fromm M (2002) Claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells. J. Cell Sci. 15(24): 4969-4976 [PubMed] [HTML] [PDF]
Amasheh S, Barmeyer C, Koch CS, Tavalali S, Mankertz J, Epple HJ, Gehring MM, Florian P, Kroesen AJ, Zeitz M, Fromm M, Schulzke JD (2004) Cytokine-dependent transcriptional down-regulation of epithelial sodium channel (ENaC) in ulcerative colitis. Gastroenterology 126: 1711-1720 [PubMed] [HTML] [PDF]
Amasheh S, Schmidt T, Mahn M, Florian P, Mankertz J, Tavalali S, Gitter AH, Schulzke JD, Fromm M (2005) Contribution of claudin-5 to barrier properties in tight junctions of epithelial cells. Cell Tiss. Res. 321(1): 89-96 [PubMed] [HTML] [PDF]
Bürgel N, Bojarski C, Mankertz J, Zeitz M, Fromm M, Schulzke JD (2002) Mechanisms of diarrhea in collagenous colitis. Gastroenterology 123(2): 433-443 [PubMed] [HTML] [PDF]
Gitter AH, Bendfeldt K, Schulzke JD, Fromm M (2000) Leaks in the epithelial barrier caused by spontaneous and TNFa-induced single-cell apoptosis. FASEB J. 14: 1749-1753 [PubMed] [HTML] [PDF]
Günzel D*, Florian P* (*shared first authorship), Richter JF, Troeger H, Schulzke JD, Fromm M, Gitter AH (2006) Restitution of single-cell defects in the mouse colon epithelium differs from that of cultured cells. Am. J. Physiol. - Reg. Integ. Comp. Physiol. [Supplemental videos] 290: R1496-R1507.
Kausalya PJ*, Amasheh S* (*shared first authorship), Günzel D, Wurps H, Müller D, Fromm M, Hunziker W (2006) Disease-associated mutations affect intracellular traffic and paracellular Mg2+ transport function of claudin-16. J. Clin. Invest. 116(4): 878-891.
Saitou M, Furuse M, Sasaki H, Schulzke JD, Fromm M, Takano H, Noda T, Tsukita S (2000). Complex phenotype of mice lacking occludin, a component of tight junction strands. Mol. Biol. Cell 11: 4131-4142.
Schmitz H, Fromm M, Bentzel CJ, Scholz P, Bode H, Epple HJ, Riecken EO, Schulzke JD (1999) Tumor necrosis factor-alpha (TNFa) regulates the epithelial barrier in the human intestinal cell line HT-29/B6. J. Cell Sci. 112: 137-146.
Schmitz H, Barmeyer C, Fromm M, Runkel N, Foss HD, Bentzel CJ, Riecken EO, Schulzke JD (1999) Altered tight junction structure contributes to the impaired epithelial barrier function in ulcerative colitis. Gastroenterology 116: 301-309.
Schulzke JD, Gitter AH, Mankertz J, Spiegel S, Seidler U, Amasheh S, Saitou M, Tsukita S, Fromm M (2005) Epithelial transport and barrier function in occludin-deficient mice. Biochim. Biophys. Acta - Biomembranes 1669(1): 34-42.
Welsh MJ, Smith PL, Fromm M, Frizzell RA (1982) Crypts are the site of intestinal fluid and electrolyte secretion. Science 218: 1219-1221.