Core Facility for ultrastructural analysis
Multi user group for transmission electron microscopy and scanning electron microscopy

Project Summary

The core facility for electron microscopy offers an ultrastructurally oriented service which may serve to perform cell biology and pathology-oriented projects within the Research Cluster FOR 667.

Transmission and scanning electron microscopical techniques (TEM, SEM) with broad application are offered. The service comprises advice in fixation protocols, tissues may also be fixed in the department, and we can also present help within scientific matter itself. This is commonly useful when the dimension of potential evaluation approaches is considered. On special request, immunoelectron microscopy can be performed using postembedding techniques (freeze substitution plastic embedding in hydrophilic resin), or preembedding techniques applying peroxidase or immunogold/silver labeling. Evaluation until the final micrograph can be done, or users can perform evaluation at the EM themselves. A modest fare per finalized specimen has been fixed that is demanded by the Charité system of ILV (internal cost allocation).

We have been specializing in cell culture of various kinds, in organ systems such as kidney, liver and intestine, lung, heart and blood vessels, and in hematology specimens. We further master the fine structural evaluation of fractions of cellular organelles, membrane structures and other. Structure preservation problems can also be advised in the latter discipline.

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