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Freie Universität Berlin Charité University Medicine Berlin Humboldt University Berlin Max-Delbrück-Center for Molecular Medicine, Berlin-Buch |
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Freie Universität Berlin Charité University Medicine Berlin Humboldt University Berlin Max-Delbrück-Center for Molecular Medicine, Berlin-Buch |
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GRK 1123: |
Cellular Mechanisms of Learning and Memory Consolidation |
This Research Training Group is funded by the German Research Council DFG
| Magnus Mayer Address: Freie Universität Berlin Biochemie, AG Multhaup Thielallee 63 D-14195 Berlin Tel +49 (30) 838-55916 |
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| PhD-Project "Zinc-dependent oligomerization of APP family proteins" Synopsis The amyloid precursor protein (APP) of Alzheimer’s disesase is part of a larger protein family that includes the amyloid precursor like proteins APLP1 and APLP2. These proteins are highly conserved and mice studies showed a strong functional redundancy among the three proteins. The physiological function of this protein family remains questionable although putative functions in metal ion transport, cell-cell or cell-matrix functions were suggested. We investigate the effects of zinc on localization of the APP family proteins and its significance on the function of these proteins in their cellular context. First analysis showed a zinc-dependent oligomerization of APLP1 and APLP2 at the plasma membrane of living cells. I employ different in vitro and in vivo techniques to investigate this effect including recombinant proteins, chromatography methods, mass spectrometry, immunofluorescence and FRET analysis in living cells and functional assays in primary neuronal culture.
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